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Interscience for microbiology
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    • Gravimetric dilutors
    • Lab blenders
    • NEWDispensing pumps
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    • Automatic platers
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Application advice

Comparison of manual and automatic methods in microbiology
Comparison of manual and automatic methods in microbiology
Why use a filter bag?
Why use a filter bag?
The Spiral plating technique
The Spiral plating technique
Sample preparation
  • Gravimetric dilutors
  • Lab blenders
  • NEWDispensing pumps
  • Lab accessories
Bacterial analysis
  • Automatic platers
  • NEWColony counters and inhibition zone readers
  • Real-time incubator and colony counter
  • NEWPetri dish labeling solution
Consumables
  • Blender bags with filter
  • Blender bags without filter
  • Blender bags with culture media
  • Sterile sampling bags
  • Autoclave deodorants
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    Application advice
    • Comparison of manual and automatic methods in microbiology
    • Why use a filter bag?
    • The Spiral plating technique
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Comparison of manual and automatic methods in microbiology

The use of an automated method for the analysis of samples increases not only reproducibility but also productivity and reduces the use of consumable up to 75%. It brings repeatability and complete traceability.

Manual method

Chronometer 16:40

Total
analysis time

Automatic method

Chronometer 5:30

Total
analysis time

75%

less
consumables

  • 1
  • 2
  • 3
  • 4
  • 5

1. Dilute the sample

OK

Automatic method

Gravimetric dilutors

Automatic dilution with DiluFlow

Automatically dilute the sample with the appropriate mass of diluent.

Chronometer 1:05

  • Fast: 225 mL in 8 s
  • Dilution accuracy: > 99% (in Standard or Accurate mode under optimal conditions)
  • Reproducible
  • Secure
  • Automatic traceability
Our gravimetric dilutors

Gravimetric dilutors

More info
Our gravimetric dilutors

Gravimetric dilutors

More info

No

Manual method

Manual dilution

Weight and dilute a the sample

Weigh the sample and manually add the diluent.

Chronometer 4:00

  • Long and tedious
  • Lack of precision
  • Not reproducible
  • Lack of security
  • Manual traceability

2. Blend the sample

OK

Manual method

Laboratory blenders

Blending the sample with BagMixer

Chronometer 2:00

  • Time saving on cleaning
  • Filtration and blending
  • No cross-contamination
  • Optimal bacterial extraction
Our laboratory blenders

Lab blenders

More info
Our laboratory blenders

Lab blenders

More info

No

Manual method

Sample grinding

Sample grinding in a blender

Chronometer 3:00

  • Time consuming cleaning
  • No filtration
  • Risk of cross-contamination
  • Undesirable cell lysis

3. Collect the filtrate

OK

Automatic method

Filter bags for blenders

Collect the filtrate in an interscience filter bag

Diluted and blended sample particles

Diluted and blended sample particles in the bag

Microorganisms to be analyzed

Microorganisms to be analyzed
 

Filter

Filter

  • Instant filtration
  • Easy pipetting
Our filter bags for blenders

Blender bags with filter

More info

Diluted and blended sample particles

Diluted and blended sample particles in the bag

Microorganisms to be analyzed

Microorganisms to be analyzed
 

Filter

Filter

Our filter bags for blenders

Blender bags with filter

More info

No

Manual method

Bag without filter

Collect the filtrate in a bag without filter

  • No filtration
  • Blocked pipette

4. Dilute and plate the sample

OK

Automatic method

Automatic platers

Automatic plating with easySpiral

Chronometer 0:25

  • From 100 to 1x107 CFU/mL on 1 Petri dish
  • Up to 75% savings in consumables
  • 75% savings on consumables automatic method

  • Automatic traceability to automatic colony counters with dataLink
Our automatic platers

Automatic platers

More info
Our automatic platers

Automatic platers

More info

No

Manual method

Manual plating

Manual serial dilutions

Chronometer 3:40

  • Repetitive action method: 4 dilutions + 4 successive platings
  • Lots of consumables
  • Consumables used with the manual method

  • Manual traceability

5. Counting the colonies

OK

Automatic method

Automatic colony counters

Simplified counting with filter bag - Correlation for automatic counting

Chronometer 2:00

  • Counting is simplified with the use of a filter bag
  • Standardized and reproducible results (Study conducted on Bacillus cereus, Escherichia coli and Lactobacillus casei.)
  • Results exported and archived automatically
Traceability export data - Connection SIL/LIMS

Connexion LIMS

 Traceability export data - 21 CFR Part 11

Audit trail

Bar-code

Bar-code

 Import data traceability - DataLink

dataLink

Traceability export data - PDF

PDF

Traceability export data - Saving and archiving sessions

Backup and archive sessions

Traceability export data - JPG

JPG, PNG, BMP

 Traceability export data - CSV

CSV

 Traceability export data

ExcelTM OpenOfficeTM

Our Automatic colony counters

Colony counters

More info
Our Automatic colony counters

Colony counters

More info

No

Manual method

Manual counting

Counting is difficult with a bag without filter - Correlation for manual counting

Chronometer 6:00

  • Difficult counting with the use of a bag without a filter
  • Variable and uncertain results
  • Results entered manually:
    error-prone and time-consuming