The analysis begins with on-site and/or on production line collection. The flexibility of our range of RollBag bags can be adapted to any type of test taking (challenge tests, cream, mascara...).

2 Dilute

2 Dilute

Weigh the sample with the dilutor. The DiluFlow Elite 1 kg dilutor automatically dispenses the diluent according to the chosen dilution factor with ± 2 % accuracy in accordance with the standards.

3 Plate

3 Plate

Place the filtered sample in a beaker. The easySpiral Dilute plater automatically dilutes and plates the sample into a Petri dish, allowing 4 logs of dilution to be made on the same dish.

4Incuber et compter

4Incubate and count

The ScanStation is an smart incubator that maintains an optimal temperature for microbial growth while analysing colony development in real time for enumeration. This real time growth monitoring allows more accurate enumeration and anticipated results. In addition, the ScanStation is 21 CFR part 11 certified, guaranteeing data protection and result traceability.

Types of analysis

The cosmetics industry uses our products for microbiological analyses of raw materials and/or in-process and finished products. Our products are also used for challenge tests in Quality Control and R&D.

The use of our products improves the quality and reliability of microbiological analyses. You will also obtain better production management.

  • Quality control
    • Enumeration of finished products
    • Challenge test
    • Enumeration of the 5 reference microorganisms:
      Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Aspergillus brasiliensis, Candida albicans.
    • Environmental control and bioburden
  • Research & Development

    Various microbiological analyses of cosmetic product before it is placed on the market.

    • Study of formulation
    • Validation process
    • Challenge test
    • Study on aging

Example of protocol in the cosmetics industry

Dilute the sample at 1/10th with DiluFlow

Weigh 1g of sample in a tube / bag and dilute to 1/10th + neutralizer with DiluFlow Elite 1 kg

Homogenize with MiniMix lab blender

Filtrate and homogenize products with many particles, with addition of polysorbate if necessary

Plate with easySpiral

Plate filtrated product for enumeration

Incubate Petri dishes with ScanStation

Place the Petri dishes in incubation in ScanStation

Obtain a compliance result with ScanStation

Enumerate colonies to obtain a compliant result with ScanStation or Scan

Dilute the sample at 1/10th with DiluFlow

Weigh 1g of artificially contaminated sample into a tube and dilute 1/10th with DiluFlow Elite 1 kg

Plate with easySpiral

Plate the sample for the challenge test enumeration

Incubate Petri dishes with ScanStation

Place the Petri dishes in incubation in ScanStation

Real-time enumeration with ScanStation

Enumerate colonies to get an accurate result with ScanStation or Scan

In compliance with international standards

ISO 11930

Evaluation of the antimicrobial protection of a cosmetic product.

European Pharmacopoeia

The European Pharmacopoeia is a unique reference work on the quality control of medicines in the countries that are signatories to the Convention relating to its elaboration.



Application notes

  • Dispensing repeatability of 9 mL from a 2-liter bottle with FlexiPump

    Aim: Evaluate the repeatability of a volume dispensed consecutively, after a FlexiPump calibration, from a FlexiPump calibration from a 2L diluent bottle (tube weight at the bottom and bottle filled for calibration).

    Conclusion: From the results obtained in this test, we can conclude that dispensing successive doses with the FlexiPump, from a 2-liter bottle of diluent, shows excellent repeatability. There is no drift in dispensing volume as the bottle empties.

  • Dispensing repeatability of 9 mL diluent bag with FlexiPump

    Aim: Evaluate the repeatability of a consecutively dispensed volume, after instrument calibration, from a 2L diluent bag.

    Conclusion: From the results obtained in this test, we can conclude that dispensing successive doses with the FlexiPump, from a diluent bag, shows excellent repeatability. There is no drift in dispensing volume as the bag empties.

  • 50 ML dispensing test with FlexiPump Pro

    Aim: Check the dispensing accuracy of a 50 mL volume with a FlexiPump Pro peristaltic pump, with a dispensing time of less than 4/5 seconds.

    Conclusion: The FlexiPump Pro is accurate for a 50 mL dispense, under the conditions described above. Note that under these conditions, each dispense took 2.7s.

  • Validation of easySpiral/easySpiral Pro plating
    1. Plating validation easySpiral/easyeasySpiral Pro in decreasing exponential mode
      1. Aim: To study the reliability of easySpiral/easySpiral Pro plating in decreasing exponential mode (known as the Spiral method) by carrying out a comparative study with the reference method "manual surface plating" (known as the classic technique).
      2. Conclusion: Our results show a strong correlation (R2 ≥ 0.982) between plating in Spiral mode using the easySpiral and the conventional method. Also, the average difference in log CFU/mL between the two methods is 12 times less than the maximum difference, and therefore not significant.
    2. Cleaning efficiency of easySpiral/easyeasySpiral Pro
      1. Aim: Ensure that the easySpiral/easySpiral Pro stylus is cleaned between each sample run. Check the effectiveness of stylus disinfection by inoculating with sterile diluent.
      2. Conclusion: The study shows that for all six bacterial strains, cleaning in either long or short mode enables disinfection of the stylus and avoids contamination between samples. Three disinfectants were tested: ethanol 70%, bleach 1% and a formulation based on hydrogen peroxide (3%) + peracetic acid (0.08%). Other formulations with slightly different concentrations of H2O2 and APA may also be effective.
  • Scan 1200: performance evaluation

    Aim: The aim of this study was to evaluate the performance of the Scan 1200 by comparing manual and automatic counting. For optimal comparison, Petri dishes were plated and incubated in our R&D laboratory, using standard methods to reproduce normal laboratory conditions. The same technician then counted the colonies with a Scan 1200 and manually to obtain results for evaluating the accuracy of the Scan. This document also contains a study of the analysis time per dish and an estimate of the time spent by the laboratories.

    Conclusion: Tests show in a variety of ways (regression line, correlation coefficient, average Log value difference, and ISO 7218:2007) that Scan 1200:
    — Enables faster counting (up to 80% time saving).
    — Counts as well as another user (strong relationship between the 2 methods, with an average difference of 2.35% per dish).

    The Scan 1200 is an excellent tool for laboratories needing to count large numbers of dishes accurately and without wasting time. All results can be saved in specific files (called sessions) which contain all dish photos and counts, guaranteeing analysis quality and perfect traceability.

  • ScanStation in cosmetic testing

    Aim: The objective of this study is to evaluate the ScanStation 100’s performances vs. manual method for the analysis of any matrices in beauty care from pure strains. For an optimal interpretation, the graph, contained in this file, is realized from 109 samples, the average of every duplicate. The maximum difference chosen in absolute value is 0.3 log

    Conclusion: The different graphs show that manual counting and counting with the ScanStation do not present significant differences except where explicable. We see that the ScanStation tends to overestimate the counting compared to manual counting; the cosmetic matrices present many colony-resembling particles, which the ScanStation differentiates better than the human eye.

  • Counting waterborne pathogens with ScanStation, a real-time incubation and counting station. Use of filtration membranes

    Aim: The objective of this study was to evaluate the performance of the ScanStation to count in real time colonies on membrane filtration. Enumeration was performed on waterborne pathogens that have been linked to healthcare-associated infections (HAIs). Bacterial suspensions were passed through filtration membranes that were deposited on Petri dish. Colonies were manually counted and results were compared with automatic counts performed by the ScanStation.

    Conclusion: ScanStation performs well in counting colonies on filtration membranes in real time. For the seven strains tested, the automatic and manual counts are similar when bacterial suspensions are filtered on white polycarbonate membranes (without grids). For best automatic counts in this case, the recommended light configuration is white background (light from below). This study shows that bacterial colonies can be efficiently counted with ScanStation.

  • Robustness tests on ScanStation

    Aim: The aim of this study is to evaluate the performance of ScanStation (ISS) by comparing manual and automatic counting of plated samples for robustness counting assessment.

    Conclusion: ScanStation’s robustness tests showed reproducible data under both intra- and inter-machine conditions.

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The different activity sectors

Our products are also used for microbiological analyses in food, pharmaceutical, environment, cosmetics, veterinary industries and public research institutes.