The analysis begins with on-site and/or on production line collection. The flexibility of our range of RollBag bags adapts to any type of test taking (biopsies, soils, faecal matter...).
Sampling for microbiological analysis preparation. The sample is placed in its unique bag with filter (BagFilter Pipet & Roll) for blender avoiding cross contamination. The bag stands upright with a BagOpen.
Weigh the sample with the DiluFlow Elite 5 kg. The DiluFlow dilutor automatically dispenses the diluent according to the chosen dilution factor with ± 2 % accuracy in accordance with the standards.
The sample is homogenized in a BagMixer SW without any contact with the blender, thus avoiding cross contamination. Bacteria are quickly extracted from the sample without being destroyed, and the filter present in the bag allows to eliminate particles in the filtrate.
Homogenized samples are closed thanks to the repositionable closing sticker of the BagFilter Pipet & Roll. They are then stored in a BagRack Slide. The pipetting area, positioned at the bottom of the bag, allows an easy sampling of the filtrate. Easily open/close the pipette access with the repositionable sticker.
With FlexiPump, the serial distribution of culture media, agars, diluents... is accurate, fast and sterile.
Place the filtered sample in a beaker.
The easySpiral Dilute plater automatically dilutes and plates the sample on a Petri dish, allowing 4 logs of dilution plated on the same dish. The easySpiral software collects the plating data.
Once the incubation is done, the automatic counting is carried out with the automatic Scan range of colony counters. Click on Click on “Count” then “Validate”. The images and results are automatically saved in your computer to ensure traceability.
From sample collection to microbial analysis, we offer you a wide range of products for all analyses in food, pharmaceutical, cosmetics, environmental, veterinary industries and public institutes research.
Collect the sample with RollBag
Weigh 25 g of sample and dilute 1/10 with DiluFlow
Homogenize the sample in a BagMixer
Plate quality indicators with easySpiral
Place the Petri dishes for incubation in ScanStation
Enumerate colonies to obtain a compliance result with ScanStation or Scan
Collect the sample with RollBag
Weigh 20 to 50 g faecal sample and dilute with DiluFlow
Homogenize and filter the sample in a BagMixer
Plate the filtrate with easySpiral using the constant mode and then place the antibiotic discs before incubation.
SIR analysis and interpretation of multi-resistance according to the CASFM veto with the Automatic Scan range
MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS
General requirements and guidance for microbiological examinations
FOOD MICROBIOLOGY
Horizontal method for the enumeration of microorganisms
The European Pharmacopoeia is a unique reference work on the quality control of medicines in the countries that are signatories to the Convention relating to its elaboration.
Following the recommendations of the WHO Expert Committee on Biological Standardisation (technical reports no. 610, 1977), the French Society of Microbiology created a Committee on Antibiogram (CA-SFM) to determine the critical values that delimit the clinical categories (previously therapeutic categories) and to propose a guide for the determination of bacterial susceptibility to antibiotics in association with EUCAST. The critical values defined for the concentrations and diameters of the inhibition zones, as well as recommendations specific to certain species or groups of antibiotics are published in this press release.
MICROBIOLOGY
General guidelines for the preparation of dilutions for microbiological examination
BACTERIOLOGICAL ANALYTICAL MANUAL
Aim: Evaluate the repeatability of a volume dispensed consecutively, after a FlexiPump calibration, from a FlexiPump calibration from a 2L diluent bottle (tube weight at the bottom and bottle filled for calibration).
Conclusion: From the results obtained in this test, we can conclude that dispensing successive doses with the FlexiPump, from a 2-liter bottle of diluent, shows excellent repeatability. There is no drift in dispensing volume as the bottle empties.
Aim: Evaluate the repeatability of a consecutively dispensed volume, after instrument calibration, from a 2L diluent bag.
Conclusion: From the results obtained in this test, we can conclude that dispensing successive doses with the FlexiPump, from a diluent bag, shows excellent repeatability. There is no drift in dispensing volume as the bag empties.
Aim: Check the dispensing accuracy of a 50 mL volume with a FlexiPump Pro peristaltic pump, with a dispensing time of less than 4/5 seconds.
Conclusion: The FlexiPump Pro is accurate for a 50 mL dispense, under the conditions described above. Note that under these conditions, each dispense took 2.7s.
Aim: The aim of this study was to evaluate the performance of the Scan 1200 by comparing manual and automatic counting. For optimal comparison, Petri dishes were plated and incubated in our R&D laboratory, using standard methods to reproduce normal laboratory conditions. The same technician then counted the colonies with a Scan 1200 and manually to obtain results for evaluating the accuracy of the Scan. This document also contains a study of the analysis time per dish and an estimate of the time spent by the laboratories.
Conclusion: Tests show in a variety of ways (regression line, correlation coefficient, average Log value difference, and ISO 7218:2007) that Scan 1200:
— Enables faster counting (up to 80% time saving).
— Counts as well as another user (strong relationship between the 2 methods, with an average difference of 2.35% per dish).
The Scan 1200 is an excellent tool for laboratories needing to count large numbers of dishes accurately and without wasting time. All results can be saved in specific files (called sessions) which contain all dish photos and counts, guaranteeing analysis quality and perfect traceability.
Aim: The aim of this study was to evaluate the performance of the ScanStation 100 by comparing the manual and automatic methods for food and milk payment analysis. For an optimal comparison, 1238 food samples, in duplicate, were carried out on a multitude of micro-organisms according to the laboratory’s reference methods. This document also contains curves showing the evolution of bacterial load over time.
Conclusion: Interpretation of these curves shows that the number of CFUs evolves up to 15 h of incubation. Thereafter, the number of CFU remains constant. Real-time counting during incubation enables us to quickly determine the presence of contamination, for example, and thus to define corrective actions before the end of incubation.
Aim: The aim of this study was to evaluate the performance of the ScanStation (ISS) by comparing manual and automatic counting on the analysis of samples seeded on Symphony and TBX media.
Conclusion: The difference in the majority of counts does not exceed the 0.3 log CFU limit. These results show no significant difference. By reading the "Time to Result" of the various microorganisms grown on Symphony and TBX media, it is possible to anticipate count results, enabling the user to define corrective action more quickly.
Aim: The aim of this study was to evaluate the performance of the ScanStation (ISS) by comparing manual and automatic decomposition of pure cultures of Salmonella typhimurium and Listeria monocytogenes.
Conclusion: The difference in the majority of counts does not exceed the 0.3 log CFU limit. These results show no significant difference. Reading the "Time to Result" for Salmonella typhimurium and Listeria monocytogenes makes it possible to anticipate count results, enabling the user to define corrective action more quickly.
Aim: The objective of this study was to evaluate the performance of the ScanStation to count in real time colonies on membrane filtration. Enumeration was performed on waterborne pathogens that have been linked to healthcare-associated infections (HAIs). Bacterial suspensions were passed through filtration membranes that were deposited on Petri dish. Colonies were manually counted and results were compared with automatic counts performed by the ScanStation.
Conclusion: ScanStation performs well in counting colonies on filtration membranes in real time. For the seven strains tested, the automatic and manual counts are similar when bacterial suspensions are filtered on white polycarbonate membranes (without grids). For best automatic counts in this case, the recommended light configuration is white background (light from below). This study shows that bacterial colonies can be efficiently counted with ScanStation.
Aim: The aim of this study is to evaluate the performance of ScanStation (ISS) by comparing manual and automatic counting of plated samples for robustness counting assessment.
Conclusion: ScanStation’s robustness tests showed reproducible data under both intra- and inter-machine conditions.
CIRI
International research centre for infectious diseases
AQMC Laboratory
Consumer Safety and Health
TERANA Public Analysis Laboratory
Services provided by the Conseil départemental du Puy-de-Dôme, France
Our products are also used for microbiological analysis in the food, environmental, pharmaceutical, cosmetic, animal health and public institutes sectors.