Green Venice High School - NIORT
Student: RONDARD Norah
Medium: Kligler with Citrobacter freundii, Serratia marcescens, Micrococcus luteus
To make this drawing I used the kligler medium (red at the beginning) because the colors I chose develop only on this medium. I seeded with the help of toothpicks to be more precise in the drawing.
I decided to use as bacteria :
❯ Citrobacter (it produces H2S at 37°C which allows to make black, and acidifies the medium which will become yellow) for the contours of the flower.
❯ The Serratia (it produces a pink pigment at 30°C) by small touches for the interior of the petals.
❯ The Micrococcus (it produces a yellow pigment at 37°C) by small touches on the top of the flower.
To obtain these colors I had to do two incubations. The first one was at 30°C for 24 h to facilitate the development of Serratia and the second one was in anaerobic conditions at 37°C for 24 h so that there is production of H2S by the Citrobacter.
The agar that I made contained a little too much water which created a strong condensation during the incubation.