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Interscience for microbiology
  • PRODUCTS
    • Sample preparation
    • Gravimetric dilutors
    • Lab blenders
    • NEWDispensing pumps
    • Lab accessories
    • Bacterial analysis
    • Automatic platers
    • NEWColony counters and inhibition zone readers
    • Real-time incubator and colony counter
    • NEWPetri dish labeling solution
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    • Blender bags with filter
    • Blender bags without filter
    • Blender bags with culture media
    • Sterile sampling bags
    • Autoclave deodorants
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Sample preparation
  • Gravimetric dilutors
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  • NEWDispensing pumps
  • Lab accessories
Bacterial analysis
  • Automatic platers
  • NEWColony counters and inhibition zone readers
  • Real-time incubator and colony counter
  • NEWPetri dish labeling solution
Consumables
  • Blender bags with filter
  • Blender bags without filter
  • Blender bags with culture media
  • Sterile sampling bags
  • Autoclave deodorants
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    • The Spiral plating technique
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Application notes

    Dispensing pumps thumbnailFlexiPump
  • Dispensing repeatability of 9 mL from a 2-liter bottle with FlexiPump
    Sectors: Food, Environment, Pharmaceutical, Cosmetic, Veterinary, Public research.

    Aim: Evaluate the repeatability of a volume dispensed consecutively, after a FlexiPump calibration, from a FlexiPump calibration from a 2L diluent bottle (tube weight at the bottom and bottle filled for calibration).

    Conclusion: From the results obtained in this test, we can conclude that dispensing successive doses with the FlexiPump, from a 2-liter bottle of diluent, shows excellent repeatability. There is no drift in dispensing volume as the bottle empties.

    Read more (PDF - 251.2 ko)
  • Dispensing repeatability of 9 mL diluent bag with FlexiPump
    Sectors: Food, Environment, Pharmaceutical, Cosmetic, Veterinary, Public research.

    Aim: Evaluate the repeatability of a consecutively dispensed volume, after instrument calibration, from a 2L diluent bag.

    Conclusion: From the results obtained in this test, we can conclude that dispensing successive doses with the FlexiPump, from a diluent bag, shows excellent repeatability. There is no drift in dispensing volume as the bag empties.

    Read more (PDF - 262 ko)
  • 50 ML dispensing test with FlexiPump Pro
    Sectors: Food, Environment, Pharmaceutical, Cosmetic, Veterinary, Public research.

    Aim: Check the dispensing accuracy of a 50 mL volume with a FlexiPump Pro peristaltic pump, with a dispensing time of less than 4/5 seconds.

    Conclusion: The FlexiPump Pro is accurate for a 50 mL dispense, under the conditions described above. Note that under these conditions, each dispense took 2.7s.

    Read more (PDF - 162.7 ko)

    Automatic Spiral platers thumbnaileasySpiral
  • Validation of easySpiral/easySpiral Pro plating
    Sectors: Food, Environment, Pharmaceutical, Cosmetic, Public research.

    1. Plating validation easySpiral/easyeasySpiral Pro in decreasing exponential mode
      1. Aim: To study the reliability of easySpiral/easySpiral Pro plating in decreasing exponential mode (known as the Spiral method) by carrying out a comparative study with the reference method "manual surface plating" (known as the classic technique).
      2. Conclusion: Our results show a strong correlation (R2 ≥ 0.982) between plating in Spiral mode using the easySpiral and the conventional method. Also, the average difference in log CFU/mL between the two methods is 12 times less than the maximum difference, and therefore not significant.
    2. Cleaning efficiency of easySpiral/easyeasySpiral Pro
      1. Aim: Ensure that the easySpiral/easySpiral Pro stylus is cleaned between each sample run. Check the effectiveness of stylus disinfection by inoculating with sterile diluent.
      2. Conclusion: The study shows that for all six bacterial strains, cleaning in either long or short mode enables disinfection of the stylus and avoids contamination between samples. Three disinfectants were tested: ethanol 70%, bleach 1% and a formulation based on hydrogen peroxide (3%) + peracetic acid (0.08%). Other formulations with slightly different concentrations of H2O2 and APA may also be effective.
    Read more (PDF - 824.6 ko)

    Automatic colony counters thumbnailScan
  • Scan 1200: performance evaluation
    Sectors: Food, Environment, Pharmaceutical, Cosmetic, Veterinary, Public research.

    Aim: The aim of this study was to evaluate the performance of the Scan 1200 by comparing manual and automatic counting. For optimal comparison, Petri dishes were plated and incubated in our R&D laboratory, using standard methods to reproduce normal laboratory conditions. The same technician then counted the colonies with a Scan 1200 and manually to obtain results for evaluating the accuracy of the Scan. This document also contains a study of the analysis time per dish and an estimate of the time spent by the laboratories.

    Conclusion: Tests show in a variety of ways (regression line, correlation coefficient, average Log value difference, and ISO 7218:2007) that Scan 1200:
    — Enables faster counting (up to 80% time saving).
    — Counts as well as another user (strong relationship between the 2 methods, with an average difference of 2.35% per dish).

    The Scan 1200 is an excellent tool for laboratories needing to count large numbers of dishes accurately and without wasting time. All results can be saved in specific files (called sessions) which contain all dish photos and counts, guaranteeing analysis quality and perfect traceability.

    Read more (PDF - 3.2 mo)

    Real-time incubator and colony counters thumbnailScanStation
  • ScanStation in the food industry
    Sectors: Food, Public research.

    Aim: The aim of this study was to evaluate the performance of the ScanStation 100 by comparing the manual and automatic methods for food and milk payment analysis. For an optimal comparison, 1238 food samples, in duplicate, were carried out on a multitude of micro-organisms according to the laboratory’s reference methods. This document also contains curves showing the evolution of bacterial load over time.

    Conclusion: Interpretation of these curves shows that the number of CFUs evolves up to 15 h of incubation. Thereafter, the number of CFU remains constant. Real-time counting during incubation enables us to quickly determine the presence of contamination, for example, and thus to define corrective actions before the end of incubation.

    Read more (PDF - 241.3 ko)
  • ScanStation in the food industry (study conducted on Symphony and TBX media)
    Sectors: Food, Public research.

    Aim: The aim of this study was to evaluate the performance of the ScanStation (ISS) by comparing manual and automatic counting on the analysis of samples seeded on Symphony and TBX media.

    Conclusion: The difference in the majority of counts does not exceed the 0.3 log CFU limit. These results show no significant difference. By reading the "Time to Result" of the various microorganisms grown on Symphony and TBX media, it is possible to anticipate count results, enabling the user to define corrective action more quickly.

    Read more (PDF - 882.1 ko)
  • ScanStation performance (study conducted with Salmonella typhimurium and Listeria monocytogenes)
    Sectors: Food, Public research.

    Aim: The aim of this study was to evaluate the performance of the ScanStation (ISS) by comparing manual and automatic decomposition of pure cultures of Salmonella typhimurium and Listeria monocytogenes.

    Conclusion: The difference in the majority of counts does not exceed the 0.3 log CFU limit. These results show no significant difference. Reading the "Time to Result" for Salmonella typhimurium and Listeria monocytogenes makes it possible to anticipate count results, enabling the user to define corrective action more quickly.

    Read more (PDF - 1 mo)
  • Real-time detection of microbial growth by the intelligent incubator to speed up environmental control processes
    Sectors: Pharmaceutical.

    Aim: ScanStation’s performance is evaluated by comparing actual manual enumeration with automatic counting of pure cultures of five microorganisms tested for environmental control in the pharmaceutical field (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus brasiliensis).

    Conclusion: In light of the results discussed in this study study, which show a strong and significant correlation between automatic ScanStation with the actual count verified by a human verified by a human operator of the set photos taken during the incubation incubation cycle, we confidently recommend confidently recommend the ScanStation for environmental environmental monitoring or analyses. In addition, time-to-result studies studies give us an insight into the growth kinetics growth kinetics of the five main pharmacopoeia strains which, if replicated and validated in the replicated and validated in the laboratory where ScanStation can be installed, to shorten the detection time for environmental and thus reduce production costs production costs in the pharmaceutical industry.

    Read more (PDF - 2.6 mo)
  • ScanStation in cosmetic testing
    Sectors: Cosmetic.

    Aim: The objective of this study is to evaluate the ScanStation 100’s performances vs. manual method for the analysis of any matrices in beauty care from pure strains. For an optimal interpretation, the graph, contained in this file, is realized from 109 samples, the average of every duplicate. The maximum difference chosen in absolute value is 0.3 log

    Conclusion: The different graphs show that manual counting and counting with the ScanStation do not present significant differences except where explicable. We see that the ScanStation tends to overestimate the counting compared to manual counting; the cosmetic matrices present many colony-resembling particles, which the ScanStation differentiates better than the human eye.

    Read more (PDF - 4 Mo)
  • Counting waterborne pathogens with ScanStation, a real-time incubation and counting station. Use of filtration membranes
    Sectors: Food, Environment, Pharmaceutical, Cosmetic, Veterinary, Public research.

    Aim: The objective of this study was to evaluate the performance of the ScanStation to count in real time colonies on membrane filtration. Enumeration was performed on waterborne pathogens that have been linked to healthcare-associated infections (HAIs). Bacterial suspensions were passed through filtration membranes that were deposited on Petri dish. Colonies were manually counted and results were compared with automatic counts performed by the ScanStation.

    Conclusion: ScanStation performs well in counting colonies on filtration membranes in real time. For the seven strains tested, the automatic and manual counts are similar when bacterial suspensions are filtered on white polycarbonate membranes (without grids). For best automatic counts in this case, the recommended light configuration is white background (light from below). This study shows that bacterial colonies can be efficiently counted with ScanStation.

    Read more (PDF - 6.8 mo)
  • Robustness tests on ScanStation
    Sectors: Food, Environment, Pharmaceutical, Cosmetic, Veterinary, Public research.

    Aim: The aim of this study is to evaluate the performance of ScanStation (ISS) by comparing manual and automatic counting of plated samples for robustness counting assessment.

    Conclusion: ScanStation’s robustness tests showed reproducible data under both intra- and inter-machine conditions.

    Read more (PDF - 313.9 ko)
  • ScanStation counting performance assessment on filtration membrane
    Sectors: Pharmaceutical, Environment, Cosmetic, Food.

    Aim: Environmental monitoring is an important part of the microbiological quality control in diverse industrial production fields (pharmaceutical, cosmetic or food). This quality control includes surfaces, air and water testing. While surface testing is mainly carried out using contact agar, air and water testing requires the use of filtration membranes to filter out microorganisms and reveal their presence. The aim of this applicative note is to assess the performance of colony counting on different types of filtration membranes.

    Conclusion: The ScanStation counting assessment results highlight that colonies are automatically detected on white membranes with correct accuracy. The ScanStation enumerates on filtration membrane with an accuracy as follow:

    • PVDF = 99%
    • MCE = 97%
    • CA = 91%
    • PES = 90%
    Read more (PDF - 565 kb)